| Authors |
Poluboyarinov Pavel Arkadyevich, Candidate of agricultural sciences, associate professor, acting head of sub-department of engineering ecology, Penza State University of Architecture and Construction (28 G. Titova street, Penza, Russia) poluboyarinovpavel@yandex.ru
Moiseeva Inessa Yakovlevna, Doctor of medical scienses, professor, head of sub-department of general and clinical pharmacology, dean of the faculty of general medicine, Penza State University (40 Krasnaya street, Penza, Russia) moiseeva_pharm@mail.ru
Glebova Natali Nikolaevna, Senior lecturer, sub-department of general and clinical pharmacology, Penza State University (40 Krasnaya street, Penza, Russia) natali.glebova@gmail.com
Chymakova Olga Aleksandrovna, Senior lecturer, sub-department of engineering ecology, Penza State University of Architecture and Construction (28 G. Titova street, Penza, Russia) ie@pguas.ru
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| Abstract |
Background. L-selenocystein (oxidized form of selenocystine) – the 21st proteinogenic aminoacid, the most significant natural selenium compound and a promising substance for correction of human selenium deficiency. An important stage of operations with l-selenocystine is the development of analytical methods of l-selenocystine identification and quantitative determination.
Materials and methods. TLC, capillary electrophoresis (Kapel 105 M) were used for detection of l-selenocystine. An elemental analysis was carried out on a vatio El cube Elementar Analysensysteme (Germany). NMR1H and 13C spectra were obtained on a Varian 400 spectrophotometer with operating frequencies of 400 and 100 MHz accordingly.
Results. The purity of l-selenocystine was evaluated using thin layer chromatography (TLC) and capillary electrophoresis. The structure of l-selenocystine was proved via the elemental analysis and 1H and 13C NMR methods.
Conclusions. As a result of the research carried out there have been developed analytical methods of l-selenocystine determination using TLC and capillary electrophoresis. The chemical structure of l-selenocystibe has proved via the elemental analysis and 1Н, 13С NMR spectrometry.
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| References |
1. Berry M. J., Banu L., Harney J. W., Larsen P. R. The EMBO Journal. 1993, vol. 12 (8),pp. 3315–3322.
2. Tutel'yan V. A., Knyazhev V. A., Golubkina N. A., Kushlinskiy N. E., Khotimchenko S. A., Sokolov Ya. A. Selen v organizme cheloveka: metabolizm, antioksidantnye svoystva, rol' v kantserogeneze [Selenium in human organism: metabolism, antioxidant properties, the role in carcinogenesis]. Moscow: Izd-vo RAMN, 2002, 224 p.
3. Metodika izmereniy massovoy doli aminokislot metodom kapillyarnogo elektroforeza s ispol'zovaniem sistemy kapillyarnogo elektroforeza «Kapel'» [The technique of measuring amino acid mass fractions by means of capillary electrophoresis using the “Kapel” capillary electrophoresis system]. OOO «Lyumeks-marketing». Saint-Petersburg, 2014, 49 p.
4. Turlo J., Gutkowska B., Malinowska E. Acta chromatographica. 2007, no. 18, pp. 36–48.
5. Poluboyarinov P. A. Mikroelementy v meditsine [Microelements in medicine]. 2015, vol. 16 (4), pp. 45–50.
6. Chocat P., Esaki N., Tanaka H., Soda K. Analytical Biochemistry. 1985, no. 148, pp. 485–489.
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