Article 4217

Title of the article



Poluboyarinov Pavel Arkadyevich, Candidate of agricultural sciences, associate professor, acting head of sub-department of engineering ecology, Penza State University of Architecture and Construction (28 G. Titova street, Penza, Russia)
Moiseeva Inessa Yakovlevna, Doctor of medical scienses, professor, head of sub-department of general and clinical pharmacology, dean of the faculty of general medicine, Penza State University (40 Krasnaya street, Penza, Russia)
Glebova Natali Nikolaevna, Senior lecturer, sub-department of general and clinical pharmacology, Penza State University (40 Krasnaya street, Penza, Russia) 
Chymakova Olga Aleksandrovna, Senior lecturer, sub-department of engineering ecology, Penza State University of Architecture and Construction (28 G. Titova street, Penza, Russia)

Index UDK

 543.61, 543.64




Background. L-selenocystein (oxidized form of selenocystine) – the 21st proteinogenic aminoacid, the most significant natural selenium compound and a promising substance for correction of human selenium deficiency. An important stage of operations with l-selenocystine is the development of analytical methods of l-selenocystine identification and quantitative determination.
Materials and methods. TLC, capillary electrophoresis (Kapel 105 M) were used for detection of l-selenocystine. An elemental analysis was carried out on a vatio El cube Elementar Analysensysteme (Germany). NMR1H and 13C spectra were obtained on a Varian 400 spectrophotometer with operating frequencies of 400 and 100 MHz accordingly.
Results. The purity of l-selenocystine was evaluated using thin layer chromatography (TLC) and capillary electrophoresis. The structure of l-selenocystine was proved via the elemental analysis and 1H and 13C NMR methods.
Conclusions. As a result of the research carried out there have been developed analytical methods of l-selenocystine determination using TLC and capillary electrophoresis. The chemical structure of l-selenocystibe has proved via the elemental analysis and 1Н, 13С NMR spectrometry.

Key words

 l-selenocystine, thin layer chromatography (TLC), capillary electrophoresis.

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Дата создания: 29.08.2017 07:58
Дата обновления: 29.08.2017 08:29